ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (3): 502-508.doi: 10.11843/j.issn.0366-6964.2014.03.023

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Study on the Expression and Localization of MATP in Different Colors of Alpaca Skin and the Correlation Analysis of Other Genes

JI Yuan-kai1,TIAN Xue1,2,FAN Rui-wen1,YU Xiu-ju1,ZHAO Yuan-yuan1,MA Shu-hui1,DONG Chang-sheng1*   

  1. (1.College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801, China; 2.College of Fisheries,Henan Normal University,Xinxiang 453007, China)
  • Received:2013-08-14 Online:2014-03-23 Published:2014-03-23

Abstract:

The objective of this study was to explore the relationship between MATP and alpaca coat color formation.Real-time quantitative PCR,western blotting and immunohistochemistry were applied for examining the mRNA and protein expression level of MATP in alpaca skin of different coat colors.The gene expression quantity of MATP in brown alpaca’s skin tissue was 3.047 times compared with white alpaca.The protein expression of MATP in brown alpaca skins was significantly higher than that in white alpaca skins(P<0.05).The MATP was demonstrated to express in epidermis,outer root sheath and dermal papilla of hair follicular by immunohistochemistry.Our findings suggested that MATP was involved in the process of alpaca coat color formation.

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